Use of phage display technology for the selection of monoclonal antibodies in allergen research and diagnostics
نویسندگان
چکیده
Background Food allergies constitutes an important global health issue for the allergic consumers, the food industry and de medical health care. Since no treatment for food allergy is yet available, the allergic patient should completely avoid the threatening allergens from his diet. This is not easy to achieve in practice because the allergic consumer can already have an allergic reaction after eating traces of relevant allergens due to cross-contamination. This is the reason why many food manufactures make more and more use of precautionary labels such as ‘may contain traces of...’ To deal with this, there is an urgent need for analytical methods than can be used in food industries which should be highly specific and sensitive, detecting traces of allergens and be rapid, robust, reliable and costeffective. For this moment, immunoassays are the most preferred method but there are only a limited number validated for only a few food allergens so research in this regard is still needed. Which allergenic compounds to detect and their change in allergenic potential during food processing are examples of important issues to be taken into consideration. The main focus of this project is the generation of relevant monoclonal recombinant antibodies against hazelnuts which are patient-dependent and/or highly specific. Hazelnuts are widely used in the food industry and belong to one of the most important food allergens.
منابع مشابه
Recombinant scFv Antibodies against P30 Surface Protein of Toxoplasma Gondii
Background & Aims: Toxoplasma gondii is an obligate, intracellular parasite, which is widely spread in the world. The parasite is able to infect all warm-blooded hosts including humans and farm animals. The infection in humans often occurs after the ingestion of raw or undercooked meat containing tissue cysts. Several methods have been applied to detect this parasite in contaminated foods. Reco...
متن کاملNanobody as a new generation of functional proteins
Nanobody (Nb) or VHH is the smallest binding domain of camelid heavy chain antibody (HcAb). Light chains of HcAb naturally removed and because of some evolutionary changes, Nbs have unique properties rather than conventional antibodies. The size of Nb is about one-tenth (0.1) of whole antibodies and this size improved some problems of four chains antibodies such as high yield of expression in p...
متن کاملPuzzling Peptides from a Phage Display Library
The commercial availability of random peptide libraries displayed on the M13 phage is increasing their use forstudies on epitope identification, enzyme inhibitors, receptor ligands, etc. In this study two experimentswhere planned for selection of peptides. First with sheep antibodies, the positive selector was IgG, preparedon Protein G column from a pool of 11 sheeps immunized...
متن کاملPhage display for the generation of antibodies for proteome research, diagnostics and therapy.
Twenty years after its development, antibody phage display using filamentous bacteriophage represents the most successful in vitro antibody selection technology. Initially, its development was encouraged by the unique possibility of directly generating recombinant human antibodies for therapy. Today, antibody phage display has been developed as a robust technology offering great potential for a...
متن کاملIsolation and Characterization of Novel Phage Displayed scFv Fragment for Human Tumor Necrosis Factor Alpha and Molecular Docking Analysis of Their Interactions
Tumor necrosis factor alpha (TNF-α) expression amplifies to excess amounts in several disorders such as rheumatoid arthritis and psoriasis. Although, Anti-TNF biologics have revolutionized the treatment of these autoimmune diseases, formation of anti-drug antibodies (ADA) has dramatically affected their use. The next generation antibodies (e.g. Fab, scFv) have not only reduced resulted immunoge...
متن کاملTargeting Colorectal Cancer Cell Lines Using Nanobodies; AgSK1as a Potential Target
Background: Colorectal cancer is the third most common type of aggressive cancers. Chemotherapy, surgery,and radiotherapy are the common therapeutic options for treating this cancer. Due to the adverse side-eff ects of these methods, immunotherapy is considered as an appropriate alternative therapeutic option. Treatment through the application of monoclonal antibodies is considered as a n...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 4 شماره
صفحات -
تاریخ انتشار 2014